Djumaeva N.E.1, Musabaev E.I.2, Meckan T.I.3

1    Acupuncture Center, Tashkent , Uzbekistan
2    Reference laboratory of Uzbekistan, Tashkent, Uzbekistan
3 Hepatologic Consultative and Diagnostic Center of the  First Municipal Hospital, Tashkent, Uzbekistan


Background: Hepatitis B is the most common cause of chronic liver disease in Uzbekistan (Central Asia). The effect of structural solutions based on  chemically pure water on chronic hepatitis B is not determined.
Objectives: To study the potential of electro-puncture diagnosis by R.Voll with the help of micro-resonance contours by V.N.Sarchuk.
To evaluate the effect and safety of structural solutions.
Design: Randomized-controlled trial.
Setting: Hepatologic Consultative and Diagnostic Center of the  First Municipal Hospital ,Reference Laboratory  of the Research Institute for Epidemioligy, Microbiology and Infectious Diseases ,Institute for Immunology  of the Academy of  Science of Uzbekistan, Acupuncture Center.(Tashkent, Uzbekistan)
Patients: 56 children with chronic hepatitis B who did not respond to medical treatment.
Intervention: 25-day course of structural solutions  in the main group and the medical treatment for 30 days in the control group.
Measurement: Biochemical, immunologic and serum measurements of hepatitis B virus ( IFA-Hbs-Ag, anti-Hbcor-JgM, anti-Hbcor-total)
Results: The study  showed  that   electro-puncture   diagnosis    by R.Voll  with  application of micro-resonance contours by V.N.Sarchuk allows to carry out  etiologic, nosologic , differential and topic diagnosis of CVHB- patients with   high sensitivity(p<0,001).The therapy with structural solutions (SS) of CVHB- patients(n=35) results to  normalization of aminotranferase from 85,7%(30) patients:  ALT 0,35?0 ,02Mm\l  compared with 2, 95?0,93Mm\l in control group(n=21),AST 0,16?0,014Mm\l compared with 1,29?0,47Mm\l in control group (p<0,05).Some immunologic  indices  was improved: T- lymphocytes ,CD-4 ,CD-16,Phagocytosis(p<0,05).Four- year  follow-up observation did not reveal differences in remissions  and exacerbations in the compared  group. However, during the 4-year  observation   Hbs-Ag  seroconversion   was reported in many children treated with SS(p< 0,001).
Conclusions: Electro-puncture diagnosis by Voll-Sarchuk technique is   non- invasive cost- effective and sensitive express- method of etiologic,  nosologic, differential and  topic diagnosis of CVHB-  patients. The therapy  with structural solutions   of   CVHB- patients provides anti-inflammatory and  immuno-modulating  effect in 85,7% of patients at the age 7-14  years.

Key words: electro-puncture diagnosis by R.Voll, micro-resonance contours by V.N.Sarchuk,  acupuncture point, meridian, structural solutions, biologically active fluid.

Chronic viral hepatitis is an urgent problem of health care due to its wide spread, development of severe forms of the disease during its exacerbation, such complications as liver cirrhosis and hepatocarcinoma (1). The disease diagnosis is based on clinical and epidemiological data, biochemical tests results, the presence of specific markers in blood serum and the results of needle biopsy of the liver (2). The patients with chronic viral hepatitis B (CVHB) are treated with the basic therapy including of anti-viral medicines, preparations of exogenic interferon or stimulants of endogenous interferonogenesis as well as desintoxicating medicines, diet, hepato-protectors, anti-oxidants, vitamins, immuno-modulators (3,4).
Recently the  electro-puncture diagnosis by R.Voll (EAV) has been used as a method of non-invasive express-diagnosis of acute viral hepatitis and of structurized solutions (biologically active fluidss) were used in therapy of patients with different diseases of inner organs (5,6). The EAV technique is   based on the ability of the body to respond to the outer exposure of the current (strength of the current is up to 1.5 microA and voltage is 1.5 –2 V) by changes in the electro-dermal resistance (EDR) in an acupuncture point (AP) in the skin. Disorders of metabolism of the organ’s tissue followed by the change of the membrane potential result in a change in the potential in the AP of the corresponding organ. This phenomenon was assumed by R.Voll as the basis of his technique (7). Analysis of the obtained readings  of the EDR is done by the value of the absolute readings of the apparatus (in conventional units) and the degree of stability of the readings during the process of measurement (“the phenomenon of the pointer’s  fall”) (8). Voll’s technique allows to evaluate the functional condition of any organ or the body’s system, to make topical, etiological, nozologic and differential diagnosis of the disease. For this purpose Voll’s technique uses the special test-agents: nozodes, hetero- and auto-antigens, organo-specific preparations, homeopathic medicines sold by the companies “Staufen-Pharma:, “Wala” and others. Preparation of test-agents is based on the principles of homeopathy.
In 1988  Ukranian scientist V.N.Sarchuk developed so-called micro-resonance contours (MRC) on the basis of the test-agents mentioned above, which are the electro-magnetic  imprinted images of different test-agents made by the special technology (9).  MRC creation has become a prerequisite for the followers of V.N.Sarchuk to develop  a new direction in medicine on the territory of the CIS, i.e. the bio-information method based on the mechanism of “imprinting”, that means  transfer of information for one object to another with the help of electro- magnetic  field characteristic(10). In V.N.Sarchuk’s technique, a MRC is a source of oscillations of the electro-magnetic field tested during EAV-diagnoses of the examinee. The substrate  processed electro-magnetically is water destructurized by distillation. V.N.Sarchuk is the author of preparation and application of so-called biologically active fluid (structural solutions)  which is chemically pure distilled water; MRC electro-magnetic images individually adjusted during examination of a patient are introduced in the solutions’ “memory”(11). High efficacy of application of these solutions was demonstrated in the therapy of patients with gastric ulcer, rheumatoid arthritis, bronchial asthma, acute viral hepatitis A, etc. (12, 13, 14).
Taking into consideration the epidemiological situation in the region of Central Asia (Uzbekistan), a great number of CVHB-patients, we have decided to study the potential of non-invasive express-diagnosis by Voll-Sarchuk and possible therapy of CVHB-patients with clinical and biochemical evidences of the disease by structural solutions.
A randomized controlled study  was carried out  to evaluate the EAV diagnostic potential and efficacy and safety of structural solutions (SS) in CVHB-patients.


The Academic Council of the Research Institute for Epidemiology, Microbiology and Infectious Diseases( RIEMID) (Health Ministry, Uzbekistan) has approved our study protocol. All patients involved in the study gave written informed consents. The study was made at the Hepatologic Consultative and Diagnostic Center (HCDC) in the First Municipal Hospital, It had been carried out in cooperation with the Reference-Laboratory of the RIEMID in the period from 1995-1999 (Tashkent, Uzbekistan). The immunologic assays were done together with the Institute for Immunology of the Academy of Sciences of Uzbekistan. The examination of the patients by the expert in EAV-diagnosis was carries out in the Acupuncture Centre.

Fifty six CVHB-patients aged 7-14 years were examined, all of them being the patients of the HCDC where the diagnosis  of CVHB was made. The duration of the disease ranged from 1  to 10 years, the process of exacerbation was reported 3-4 times per year. The duration of the last exacerbation accompanied by ensymemia (aminotranspherase) had been observed before the start of the study  for two months and longer. All the patients had received the routine basic therapy but had no response or  short-term clinical effect. Forty-seven patients (84.3%) had positive Hbs-Ag (“Orto-diagnosticum system”, USA). In other patients, the presence of Hbs-Ag was recorded retrospectively. The CVHB-diagnosis was made on the basis of serological tests (Hbs-Ag, anti-Hbcor-total, anti-Hbcor    Ig M), the findings obtained by ultrasonic examination, clinical, epidemiological, biochemical and immunologic examinations. During the treatment the aminotranferase activity (aspartat-alanin-aminotransferase), bilirubin (total and fractions) was determined. The thymol test was performed and total protein and its fractions (albumin, globulin) were determined in blood serum; hemogrammes were made. The study of the immunologic status included count of the number of leukocytes, lymphocytes, phagocytosis, immunoglobulin as well as receptors CD3, CD4, CD8, CD16.
To make the etiologic, nosologic, differential acupuncture diagnosis of CVHB, the  MCR-nozodes were used: “Hbs-Ag”, “viruses of hepatitis B,C,A,D”, “chronic active hepatitis”, “chronic persisting hepatitis”, “liver cirrhosis”, “cholecystitis”,MRC organospesific preparation of the liver, MRC of organs of the endocrine system, MRC of “mesenchimal reactivation”. The study was made in the APs in the following meridians: Liver, Spleen, Large Intestine, and Small Intestine, Triple Energizer Meridian, meridians of Connective Tissue Degeneration. The readings evaluation was made according to the accepted recommendations (R.Voll, 1978). The obtained findings were compared to already known results of the patients’ examination in the HCDC.
In the process of examination of every patient by Voll-Sarchuk technique the set of SS-prescription was made. It contained the  MRC of nozodes of “viral hepatitis B”, “chronic hepatitis”, “cholecystitis”, “Hbs-Ag”, organospecific preparations of the liver, pancreas, ileum and colon, bile ducts, spleen, lymph nodes, thymus, adrenal glands, as well as the MRC of homeopathic preparations: Carduus marianus, Berberis aquifolium, Chelidonium, Belladonna, Lachesis, Apis and others. The combinations of prescriptions was selected strictly individually. If it was necessary, the additionally tested  MRC were introduced. The SS were prepared according to the special technology (15) after examination of a patient made by an EAV- physician expert. The patients from the main group were informed on the SS storage and administration conditions (it should be kept in a thermos bottle). The dose of the medicine was 25 ml.; the administration was performed according to the recommendations: first five days – 3 times a day, the next five days – twice a day, the next 15 days - once a day, one hour before meals. The SS were prepared during the therapy course: every five-seven days for each patient. The patients from the control group were treated traditionally by basic medicines including desintoxicating drugs, hepato-protectors, immuno-modulators (Thymolin, Russia; Immunomodulin, Uzbekistan), vitamins of groups B and C, holekynetics, herbs and diet.

Statistical  Analysis

Point estimates  and 95%  CIs  were  used for all comparisons. The analysis of the obtained finding was made by the method of variation statistics by Fisher-Student, the correlation analysis was made by the method of I.A.Oivin.


Fifty-six patients, 35 in the main group and 21 in the control one, who did not respond to the traditional therapy were involved in our study. All the patients treated with SS had suffered from chronic hepatitis (12 patients were ill with chronic persisting hepatitis, 23 – chronic active hepatitis). In the control group, the patients were ill with chronic hepatitis (8 had suffered from chronic persisting hepatitis, 13 – chronic active hepatitis) (P>0.05). The diagnosis was made according to the clinical criteria of the International Classification of chronic viral hepatitis adopted in 1974 in Acapulko (16).
The basic clinical characteristics of all the patients are shown in Table 1.

Baseline Clinical Characteristics of Study Patients with CHVB (tabl.1)


Main group (n=35)

Control group

P Value

Mean age(7-14)
















ALT (mlm/l)




AST (mlm/l)




Tymol  index








?- Globulin (%)




Ò-lymphocytes (%)




ÑÄ4 (%)




ÑÄ8 (%)
















ALT- alanine aminotransferase, AST- aspartate aminotransferase
*  - authenticity difference   concerning  normal  means(p<0.05).


 The groups under study did not differ in age, sex, elevated aminotransferase level, the presence of Hbs-Ag, manifestation of clinical symptoms, the severity of the disease. The duration of the SS therapy was 25 days in the main group while in the control group, the patients had been treated for  30,04±1.21 days.
When comparing the results of the diagnosis made by Voll-Sarchuk technique to the data of the HCDC, the following findings were obtained: the presence of Hbs-Ag was determined by Voll-Sarchuk technique in 49 patients (30 patients in the main group – 85.7% and 19 controls – 90.5%) while the routine diagnostic methods revealed 47 patients (29 patients in the main group – 82.6% and 18 controls – 85.7%) (P>0.05). The diagnosis of chronic persisting hepatitis (CPH) was made   by Voll techique in 11 (31.5%) patients in the main group and in 9 (42.8%) controls. The routine diagnostic methods   revealed CPH in 12 patients (34.3%) of the main group and in 8 controls (38.1%) (P>0.05). The diagnosis of chronic active hepatitis (CAH) was made by Voll technique in 24 patients (68.5%) and in 12 controls (57.2%). The traditional diagnostic methods revealed CAH in 23 patients (65.7%) in the main group and in 13 controls (61.9%) (P>0.05). These finding demonstrate high sensitivity of Voll-Sarchuk technique in diagnosis of CVHB.
We have studied the dynamics of the major clinical symptoms of the disease, the data of laboratory, serological and immunologic tests, as well as the data of EAV diagnosis for the purpose of evaluation of efficacy of the SS therapy. The analysis showed that 30 patients of the main group (85.7%) responded positively to the SS therapy that manifested as disappearance of complaints of general malaise, fatigue, headaches, hidrosis, sense of heaviness in the right sub costal area, nausea, loss of appetite by 5.1±45 – 13.2±03 the disease days, while in the control group these had been recorded by 9±0.9 - 24.6 ±0.4 days of the disease (P<0.01 – 0.001).
The dynamics of the laboratory tests findings during the treatment is shown in Table 2.
Biochemical and Immunology Response After Treatment From Patients with CHVB ( tabl.2)



The main group
(n =30)

The control group (n=21)


P Value

After treatment

After treatment

ALT (mlm/l)




AST (mlm/l)


1,29*( 0,09-2,5)


Tymol index




Albumin (%)

60,9( 58,7-63,1)



?- Globulin (%)

18,6( 18,4-18,8)



Ò-lymphocytes (%)

66,5 (65, 2- 67, 8)

42, 6* (32, 1- 53,1)



1940 ?15

971 ?93


B-lymphocytes (%)

16, 10(18, 3-23, 4)

20, 8 (13, 9-32, 8)



607 ?29

474? 62


ÑD4 (%)

29,4* (28, 6-30, 2)

23, 4* (13,9- 32, 8)



858? 9



ÑD8 (%)

17,3 (15,2-19 ,4)

17,3 2(10,1- 24, 5)




394 ?63



13,6 (12,1-15,1)

17,5 (6,8-28,2)



397? 18




1,64 (0,86—2,41)

1,4* (1,01- 1, 79)



52,5* (48, 5-56, 5)

48,5* (36, 2-60, 8)


*  - authenticity difference   concerning  normal  means(p<0.05).

It demonstrates the positive SS influence on the course of CVHB, a reliable decrease of the indicators ALT and AST to the normal level was observed in the patients treated with the SS in comparison to the control group (P<0.05).  The thymol test values and the gamma-globulin level were reliably reduced to normal ones (P<0.05), the immunologic indicators also improved: in the group of children treated with the SS, the number of T- and B-lymphocytes normalized (both in the absolute and relative indicators). The indicators of non-specific immunologic defense improved, those of  neutrophiles phagocytic activity and CD-16 normalized (NPA) in comparison to the controls (P<0.05). We also analyzed the SS-effect on the disease course in the patients of the main group who previously   had different ALT indices. The findings are shown in Table 3 where they are compared to the ALT-indices in the controls. The table demonstrates that the effectiveness of the SS-therapy of  CVHB-patients does not depend on the previous ALT-indices. In the main patients group with different ALT- activity indices,  the SS therapy normalizes them.

The dynamics of the ALT-indices changes under the influence of the administered therapy in the compared groups of children.
1.                                                                        2.
virus1    virus2

  • In the group of patients with the ALT-level less than 1.05 Mm/l (P>0/05).
  • In the group of patients with the ALT-level from 1.05 Mm/l to 6.0 Mm/l (P<0.001).
  • In the group of patients with the ALT-level more than 6.0 Mm/l (P>0/05).


 Just after the therapy-course,  Hbs-Ag seroconversion was insignificant: in the main group it was reported in two patients (5.7%) and in two patients in the control group (9.5%). Improvement of the clinical condition and normalization of the values of the clinical and biochemical tests after the SS application had been recorded at different time: by 25 days – in 23 patients (65.7%), by 35 days – in 5 patients (14.3%), over 40 days – in 3 patients (8.6%). In our opinion it was due to the original ALT-indices values.
Analysis of the findings obtained during the study of the functional condition of organs and systems of the body by Voll’s technique demonstrated  a direct correlation  between the activity of the process and the findings of the EAV-diagnosis. A  decrease of the index of the absolute values (in conventional units) and the index of the “pointer’s fall phenomenon” in the AP meridians revealed by the Voll’s apparatus  was recorded in 58% - 86% of cases in different meridians (the Pericardium, the Connective tissue degeneration, the Liver, the  Large and small  intestines, the Spleen meridian, the Thiple Energizer meridian, the Gall-bladder meridian). There was no SS-therapy effect in 5 patients (14.3%). The SS did not cause anyadverse effect and complications.
The follow-up examination  of 33 patients of the main group (94.3%) and 15 patients of the control group (71/4%) was made in 6 months, 1, 2, 3, 4 years. Two patients of the main group and six patients of the control group were excluded from the follow-up examination because of the following reasons: 6 patients moved to another place, 1 had suffered from the developing pulmonary tuberculosis, 1 had astenic syndrom.
During this period 19 patients (57.6%) in the main group and 7 patients in the control group (46.7%) (P>0.05) showed long remission of the process verified by the biochemical tests. Six children (18.1%) in the main group and 5 (33%) in the control group  (P>0.05)  had a single exacerbation of the process  in the period  3 – 6 months against a background of accompanying diseases (chronic tonsillitis, chronic bronchitis, chronic cholecystitis, chronic pancreatitis). Eight patients (24.2%) treated with the SS and 3 patients (20%) in the control group (P>0.05) had multiple exacerbation of the process against a background of accompanying diseases verified by the corresponding clinical,  biochemical and serological tests. For 5-year follow-up period 21 patient of the main group (63.6%) and 3 patients of the control group (20%) (P<0.001) showed seroconversion of Hbs-Ag verified by serological tests (Orto-diagnosticum system.,USA).


Our study demonstrated  high sensitivity of tests by Voll’s technique and the micro-resonance contours by Sarchuk in CVHB-diagnosis. In 1990 the similar study by Voll-Sarchuk technique were made by K.O.Solovjev  (12), he diagnosed duodenal ulcers and revealed Helicobacter pylori. The sensitivity of the diagnostic method was 87.5% (40 patients). The similar    study  was done by O.N.Popovich in 1991(17) who diagnosed HIV-infection in 172 blood serum samples and in 14 HIV-patients. The sensitivity was 100% (in comparison with the reaction of immunoblotting and polymerase-chain reaction). A.S.Klyap diagnosed acute viral hepatitis A, the sensitivity was 93.3% (15 patients) ( 14).
The process of diagnostic tests performance in Voll’s technique is called “medicament testing”. It should be stressed that the test is a rather complex procedure and requires  certain skills and experience (18). The advantage of the method is its high sensitivity, cost-effectiveness (the time of MRC-use with their multiple application is 3 years), the method is non-invasive and safe, the time consumed for examination of one patient (40-60 min),an individual approach to diagnosis.
Before us Structural Solutions were not recommended and were not used to treat CVHB-patients. I believe that in different pathologic processes are going on changes  in the structure of  tightly-connected  water occur in cellular  structures of the body. The  tightly-connected intracellular water is an intracellular water which is in chemical combination with ions, bio-major-molecules of protein and other substances. The importance of structural organization of the water for inter- and extra-cellular fluids had been studied by T.G.Wotterson (19), S.V.Zenin (20) and others. The unique role of water manifests itself not only in the fact that it is a specific medium for biological reactions. It is a necessary part of protein, nucleic acids, it directly influences the formation and stabilization of the structure of biopolymers’ macromolecules and more complex permolecular compounds.
The aspectes of the structural condition of the intracellular  water in some diseases were studied  (21). According to the data of E.L.Andronikoshvily, who used nuclear magnetic resonance, in malignant cells, the time of spin-lattice relaxation changes, the amount of water bound with DNA increases, thermodynamic characteristics of the system “DNA-bound water” changes. The time of protons relaxation changes in the water solution of normal tissues of the  tumor bearing body, too. The changes in the structural condition of the bound water in cells during pathologic conditions seems to be closely associated with biochemical and biophysical changes in cellular-tissue structures.
Structural Solutions (SS) are a structural informative fluid with homeo-isopathic characteristics of test-agents, their MRC were used to prepare the SS (22). In our opinion, the SS therapeutic effect is due to its action to the structure of the tightly-connected  water  in the patient’ body by the principle of resonance or by “the law of similarity” it restores the structural condition of the tightly-connected  water  disordered by the pathologic process in injured organs and tissues. This, in turn, results in normalization of the course of biophysical and biochemical processes in them, that, hence, influences the results of clinical-biochemical and immunologic tests.
Our conception on the SS effect differs from that of  V.N.Sarchuk, the author of the technique. He believes that the mechanism of action of biologically active fluid (SS) is based on the principle of interference when an interaction of antiphase vibration with equal amplitude (two coherent sources) with the following total or partial extermination of the oscillation. This results in a change  in the energy and  aggregate condition of liquid crystal structures  up to their destruction with the following discharge from the body (23). The phenomenon of “imprinting”, i.e. transfer of information from one place to another is based on the ability of water “to remember” information. The problem of water “memory” was studied by J.Benveniste (24), G.N.Shangin-Berezovsky (25), V.P.Yamskova (26). The presence of electromagnetic field is a necessary condition of “imprinting” due to which water is structurized. The water obtained the structurized condition becomes an intermediary in biological information transfer. As SS contain no molecules of an active substance, they are pseudo-solutions.
Our investigations had some restrictions: we were not able to do biopsies of the liver because our patients did not agree to this diagnostic procedure. However, it should be mentioned that O.K.Solovjev who used Voll-Sarchuk technique, showed that the sensitivity of the diagnostic method was 85% (40 patients). The study  was made by double-blind method with verification by gastrofibroscopy (12). At the time of the study (1995-1999) we did not have an opportunity to compose the control group of patients treated with interferon-therapy because it is very expensive. However, according to the literature data the positive effect of antiviral drugs, for example, recombinant interferon-a2?, is 20-40% under the condition of its  use  not less than 4 months (16).According to the findings of  Faisullaeva D.B. (Uzbekistan) the efficacy of interferon –application is   20  % with the course duration 6-   month(27). SS high effectiveness was demonstrated in therapy of the following diseases: in 80% of cases of gastric ulcer(12), in 100% of patients with rheumatism, in 85% of patients with rheumatoid polyarthritis (13), 88.4% of cases of chronic obstructive bronchitis (28). The advantage of SS use in therapy is the absence of complications and adverse effects.
Thus, the study showed that the method of electro-puncture diagnosis by R.Voll with application of micro-resonance contours by V.N.Sarchuk is non-invasive, cost-effective, and sensitive express-method of etiologic, nosologic, differential and topic diagnosis of CVHB-patients. The obtained findings demonstrate that the results of Voll- Sarchuk  method examination  are   confirmed    by the  routine  clinical laboratory tests.  The high information capacity of Voll-Sarchuk technique was demonstrate.The therapy with structural solutions at the stage of clinical and biochemical manifestation of CVHB provides anti-inflammatory and immuno-modulating effect in 87.5% of patients at the age 7-14 years that is expressed in normalization of clinico-biochemical and some immunologic indices. Five-year follow-up observation did not reveal differences in remissions and exacerbations in the compared group (P>0.05). However, during this 5-year observation Hbs-Ag seroconversion was reported in many children treated with SS (P<0.001). We associate this with immuno- modulating   effects   of   SS .


  • The author thank M.V.Zalyalieva, Candidate of Medical Sciences, the researcher of the Institute for Immunology, the Academy of Sciences, Uzbekistan. Special thank goes to Tatiana Metskan (MD, PhD), staff of Tashkent city Hepathology Centre for constant consulting and support to the author.



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Medicale snake

Dr. Naylya Djumaeva

MD,PhD(candidate of the medical sciences), Neurologist of higher category

e-mail : dr_djumaeva@hotmail.com

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“this scientific work is relevant  to very important  problem  of resonance geometry of  processes of   individual  life activity   and  condition  of  his health and disease” Academician  of Russian  Academy of Medical Science, professor  V.P.Kaznacheev

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